ABSTRACT
Background and Objectives: The studies on relationship between different phases of menstrual cycle and pulse rate and Sp02 changes in healthy women of Kerala are inadequate. So, this preliminary study was plan to report the same. Material and Methods: The present study was performed in twenty healthy women of Kerala, age ranging from 20-40 (mean age 27+/- 61054). Spo2 and pulse rate were recorded with pulse - oximeter in different phases of menstrual cycle and analysis of data was done by spss 20.0. Results: Variations of pulse rate and Spo2 in different phases of menstrual cycle are not statistically significant at p > 0.05. Conclusion: it was observed that there was slight increase in pulse rate in ovulatory phase and slight increase in Sp02 in secretory phase, though it is not statistically significant. It may be due to small sample size. Hence this study certainly merits continuing the work with more investigations in the field.
ABSTRACT
Carriers of bovine anaplasmosis in Northern Kerala, South India were detected using conventional microscopical and molecular techniques. PCR-RFLP and nested PCR techniques were used for detection of Anaplasma marginale and Anaplasma bovis respectively and the PCR products were confirmed by sequencing. Out of 150 samples tested, 25 were detected positive for A. marginale and five for A. bovis based on molecular tests. The inclusion bodies of A. marginale could be detected by microscopy in two blood smears after staining by giemsa while acridine orange staining detected three smears positive. The data clearly suggest the higher sensitivity of molecular techniques for diagnosis of these diseases.